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@@ -558,6 +558,7 @@ status open
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\align center
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\begin_inset Graphics
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filename graphics/CD4-csaw/rulegraphs/rulegraph-all.pdf
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+ lyxscale 50
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width 100theight%
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\end_inset
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@@ -1512,6 +1513,7 @@ Comparison of quantification between Salmon with and without Shoal for identical
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Ultimately selected shoal as quantification, Ensembl as annotation.
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Why? Running downstream analyses with all quant methods and both annotations
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showed very little practical difference, so choice was not terribly important.
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+ Prefer shoal due to theoretical advantages.
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To note in discussion: reproducible workflow made it easy to do this, enabling
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an informed decision.
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\end_layout
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@@ -2648,6 +2650,270 @@ noprefix "false"
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Effect of promoter coverage upstream vs downstream of TSS
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\end_layout
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+\begin_layout Standard
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
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+\begin_layout Plain Layout
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+\align center
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+\begin_inset Graphics
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+ filename graphics/CD4-csaw/ChIP-seq/H3K4me2-neighborhood-clusters-CROP.png
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+ lyxscale 25
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+ width 100col%
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+ groupId colwidth-raster
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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+\series bold
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:H3K4me2-neighborhood-clusters"
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+
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+\end_inset
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+
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+RNA-seq PCoA showing principal coordiantes 2 and 3.
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Standard
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
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+\begin_layout Plain Layout
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+\align center
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+\begin_inset Graphics
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+ filename graphics/CD4-csaw/ChIP-seq/H3K4me2-neighborhood-PCA-CROP.png
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+ lyxscale 25
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+ width 100col%
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+ groupId colwidth-raster
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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+\series bold
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:H3K4me2-neighborhood-pca"
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+
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+\end_inset
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+
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+RNA-seq PCoA showing principal coordiantes 2 and 3.
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Standard
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
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+\begin_layout Plain Layout
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+\align center
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+\begin_inset Graphics
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+ filename graphics/CD4-csaw/ChIP-seq/H3K4me2-neighborhood-expression-CROP.png
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+ lyxscale 25
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+ width 100col%
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+ groupId colwidth-raster
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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+\series bold
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:H3K4me2-neighborhood-expression"
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+
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+\end_inset
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+
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+RNA-seq PCoA showing principal coordiantes 2 and 3.
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Standard
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
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+\begin_layout Plain Layout
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+\align center
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+\begin_inset Graphics
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+ filename graphics/CD4-csaw/ChIP-seq/H3K4me3-neighborhood-clusters-CROP.png
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+ lyxscale 25
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+ width 100col%
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+ groupId colwidth-raster
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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+\series bold
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:H3K4me3-neighborhood-clusters"
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+
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+\end_inset
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+
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+RNA-seq PCoA showing principal coordiantes 2 and 3.
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Standard
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
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+\begin_layout Plain Layout
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+\align center
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|
+\begin_inset Graphics
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+ filename graphics/CD4-csaw/ChIP-seq/H3K4me3-neighborhood-PCA-CROP.png
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+ lyxscale 25
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+ width 100col%
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+ groupId colwidth-raster
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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+\series bold
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|
+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:H3K4me3-neighborhood-pca-1"
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+
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+\end_inset
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+
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+RNA-seq PCoA showing principal coordiantes 2 and 3.
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Standard
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|
+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
|
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+\begin_layout Plain Layout
|
|
|
+\align center
|
|
|
+\begin_inset Graphics
|
|
|
+ filename graphics/CD4-csaw/ChIP-seq/H3K4me3-neighborhood-expression-CROP.png
|
|
|
+ lyxscale 25
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|
|
+ width 100col%
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+ groupId colwidth-raster
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
|
|
|
+
|
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|
+\series bold
|
|
|
+\begin_inset CommandInset label
|
|
|
+LatexCommand label
|
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+name "fig:H3K4me3-neighborhood-expression-1"
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+
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+\end_inset
|
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+
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+RNA-seq PCoA showing principal coordiantes 2 and 3.
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|
+\end_layout
|
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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\begin_layout Itemize
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H3K4me peaks seem to correlate with increased expression as long as they
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are anywhere near the TSS
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