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@@ -12522,7 +12522,7 @@ begin{landscape}
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placement p
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wide false
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sideways false
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-status collapsed
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+status open
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\begin_layout Plain Layout
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\align center
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@@ -14423,18 +14423,25 @@ Future Directions
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\end_layout
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\begin_layout Standard
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-\begin_inset Flex TODO Note (inline)
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-status open
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-
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-\begin_layout Plain Layout
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-I've already done a good bit of work outside just this globin blocking thing,
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- so I'm not sure what to put for future directions.
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- Does it inculde the other stuff I've done but not published?
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+One drawback of the globin blocking method presented in this analysis is
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+ a poor yield of genic reads, only around 50%.
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+ In a separate experiment, the reagent mixture was modified so as to address
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+ this drawback, resulting in a method that produces an even better reduction
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+ in globin reads without reducing the overall fraction of genic reads.
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+ However, the data showing this improvement consists of only a few test
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+ samples, so the larger data set analyzed above was chosen in order to demonstra
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+te the effectiveness of the method in reducing globin reads while preserving
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+ the biological signal.
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\end_layout
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-\end_inset
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-
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-
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+\begin_layout Standard
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+The motivation for developing a fast practical way to enrich for non-globin
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+ reads in cyno blood samples was to enable a large-scale RNA-seq experiment
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+ investigating the effects of mesenchymal stem cell infusion on blood gene
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+ expression in cynomologus transplant recipients in a time course after
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+ transplantation.
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+ With the globin blocking method in place, the way is now clear for this
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+ experiment to proceed.
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\end_layout
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\begin_layout Chapter
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@@ -14462,7 +14469,7 @@ status collapsed
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\begin_layout Plain Layout
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-% Call it "References" instead of "Bibliography"
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+% Use "References" as the title of the Bibliography
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\end_layout
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\begin_layout Plain Layout
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