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@@ -6,19 +6,19 @@
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## Organ transplants are a life-saving treatment for many
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## Organ transplants are a life-saving treatment for many
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-\Large
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+\LARGE <!-- This should probably be my default bullet point size -->
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-* 36,528 transplants performed in the USA in 2018[^1]
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+::: incremental
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-. . .
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+* 36,528 transplants performed in the USA in 2018[^1]
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* 100 transplants every day!
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* 100 transplants every day!
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-. . .
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-
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* Over 113,000 people on the national transplant waiting list as of
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* Over 113,000 people on the national transplant waiting list as of
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July 2019
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July 2019
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+:::
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+
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[^1]: Source: https://www.organdonor.gov/statistics-stories/statistics.html
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[^1]: Source: https://www.organdonor.gov/statistics-stories/statistics.html
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## Organ transplants are a life-saving treatment for many
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## Organ transplants are a life-saving treatment for many
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@@ -29,23 +29,25 @@
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## Rejection is an adaptive immune response against a graft
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## Rejection is an adaptive immune response against a graft
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+<!-- TODO: Need a graphic for this -->
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+
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+\LARGE
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+
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+::: incremental
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+
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* The host's adaptive immune system identifies and attacks cells
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* The host's adaptive immune system identifies and attacks cells
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bearing non-self antigens
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bearing non-self antigens
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-. . .
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-
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* An allograft contains differnet genetic variants from the host,
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* An allograft contains differnet genetic variants from the host,
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resulting in protein-coding differences
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resulting in protein-coding differences
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-. . .
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-
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* Left unchecked, the host immune system eventually notices these
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* Left unchecked, the host immune system eventually notices these
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alloantigens and begins attacking (rejecting) the graft
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alloantigens and begins attacking (rejecting) the graft
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-. . .
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-
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* Rejection is the major long-term threat to organ allografts
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* Rejection is the major long-term threat to organ allografts
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+:::
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+
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## Allograft rejection remains a major long-term problem
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## Allograft rejection remains a major long-term problem
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![Kidney allograft survival rates in children by transplant year[^3]](graphics/presentation/kidney-graft-survival.png){ height=65% }
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![Kidney allograft survival rates in children by transplant year[^3]](graphics/presentation/kidney-graft-survival.png){ height=65% }
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@@ -54,6 +56,13 @@
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## Rejection is treated with immune suppressive drugs
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## Rejection is treated with immune suppressive drugs
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+<!-- TODO: Need a graphic, or maybe a table of common drugs +
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+mechanisms, or a diagram for periodic checking. -->
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+
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+\LARGE
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+
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+::: incremental
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+
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* To prevent rejection, a graft recipient must take immune suppressive
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* To prevent rejection, a graft recipient must take immune suppressive
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drugs for the rest of their life
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drugs for the rest of their life
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@@ -62,24 +71,20 @@
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* Immune suppression is a delicate balance: too much leads to immune
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* Immune suppression is a delicate balance: too much leads to immune
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compromise; too little leads to rejection.
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compromise; too little leads to rejection.
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-
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-. . .
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-
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-* Both diagnosis and treatment present significant challenges
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-
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-* Immune memory is the major contributor to long-term rejection
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+
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+:::
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## My thesis topics
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## My thesis topics
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-### Chapter 2
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+### Topic 1: Immune memory
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Genome-wide epigenetic analysis of H3K4 and H3K27 methylation in naïve
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Genome-wide epigenetic analysis of H3K4 and H3K27 methylation in naïve
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and memory $\mathsf{CD4}^{+}$ T-cell activation
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and memory $\mathsf{CD4}^{+}$ T-cell activation
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-### Chapter 3
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+### Topic 2: Diagnostics for rejection
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Improving array-based diagnostics for transplant rejection by
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Improving array-based diagnostics for transplant rejection by
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optimizing data preprocessing
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optimizing data preprocessing
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-### Chapter 4
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+### Topic 3: Blood profiling during treatment
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Globin-blocking for more effective blood RNA-seq analysis in primate
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Globin-blocking for more effective blood RNA-seq analysis in primate
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animal model for experimental graft rejection treatment
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animal model for experimental graft rejection treatment
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@@ -94,6 +99,8 @@ and memory $\mathsf{CD4}^{+}$ T-cell activation
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## Memory cells: faster, stronger, and more independent
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## Memory cells: faster, stronger, and more independent
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+<!-- TODO: Implement progressive reveal -->
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+
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## Memory cells are a problem for immune suppression
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## Memory cells are a problem for immune suppression
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