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@@ -107,7 +107,7 @@ todonotes
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\quotes_style english
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\quotes_style english
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\dynamic_quotes 0
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\dynamic_quotes 0
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\papercolumns 1
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\papercolumns 1
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-\papersides 2
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+\papersides 1
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\paperpagestyle default
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\paperpagestyle default
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\tracking_changes false
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\tracking_changes false
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\output_changes false
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\output_changes false
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@@ -3648,7 +3648,7 @@ For the figures in this section, the group labels are arbitrary, so if time
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways true
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sideways true
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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@@ -3815,17 +3815,7 @@ kbp downstream, and the logCPM values were normalized within each promoter
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\series bold
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\series bold
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\series default
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\series default
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-and the average bin values were plotted for each cluster (
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:H3K4me2-neighborhood-clusters"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-).
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+and the average bin values were plotted for each cluster (a).
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The
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The
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\begin_inset Formula $x$
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\begin_inset Formula $x$
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\end_inset
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\end_inset
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@@ -3847,29 +3837,9 @@ shape
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of the promoter coverage for promoters in that cluster.
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of the promoter coverage for promoters in that cluster.
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PCA was performed on the same data, and the first two principal components
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PCA was performed on the same data, and the first two principal components
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- were plotted, coloring each point by its K-means cluster identity (
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:H3K4me2-neighborhood-pca"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-).
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+ were plotted, coloring each point by its K-means cluster identity (b).
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For each cluster, the distribution of gene expression values was plotted
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For each cluster, the distribution of gene expression values was plotted
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- (
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:H3K4me2-neighborhood-expression"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-.
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+ (c).
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\end_layout
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\end_layout
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\end_inset
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\end_inset
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@@ -3886,7 +3856,7 @@ noprefix "false"
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways true
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sideways true
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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@@ -4052,17 +4022,7 @@ kbp downstream, and the logCPM values were normalized within each promoter
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\series bold
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\series bold
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\series default
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\series default
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-and the average bin values were plotted for each cluster (
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:H3K27me3-neighborhood-clusters"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-).
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+and the average bin values were plotted for each cluster (a).
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The
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The
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\begin_inset Formula $x$
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\begin_inset Formula $x$
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\end_inset
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\end_inset
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@@ -4084,29 +4044,9 @@ shape
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of the promoter coverage for promoters in that cluster.
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of the promoter coverage for promoters in that cluster.
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PCA was performed on the same data, and the first two principal components
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PCA was performed on the same data, and the first two principal components
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- were plotted, coloring each point by its K-means cluster identity (
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:H3K27me3-neighborhood-pca"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-).
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+ were plotted, coloring each point by its K-means cluster identity (b).
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For each cluster, the distribution of gene expression values was plotted
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For each cluster, the distribution of gene expression values was plotted
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- (
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:H3K27me3-neighborhood-expression"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-.
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+ (c).
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\end_layout
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\end_layout
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\end_inset
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\end_inset
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@@ -4926,7 +4866,7 @@ literal "false"
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\begin_inset Float table
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\begin_inset Float table
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wide false
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wide false
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sideways false
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sideways false
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-status collapsed
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+status open
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\begin_inset Tabular
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\begin_inset Tabular
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@@ -6191,6 +6131,13 @@ fRMA with custom-generated vectors enables normalization on hthgu133pluspm
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\begin_layout Standard
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\begin_layout Standard
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\begin_inset Float figure
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\begin_inset Float figure
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+wide false
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+sideways false
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+status collapsed
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+
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+\begin_layout Plain Layout
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+\align center
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+\begin_inset Float figure
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placement tb
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placement tb
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wide false
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wide false
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sideways false
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sideways false
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@@ -6200,6 +6147,9 @@ status collapsed
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/frma-pax-bx/batchsize_batches.pdf
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filename graphics/frma-pax-bx/batchsize_batches.pdf
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+ lyxscale 50
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+ width 100col%
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+ groupId frmatools-subfig
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\end_inset
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\end_inset
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@@ -6218,13 +6168,8 @@ name "fig:batch-size-batches"
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\series bold
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\series bold
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-Effect of batch size selection on number of batches included in fRMA probe
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- weight learning.
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-
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-\series default
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-For batch sizes ranging from 3 to 15, the number of batches with at least
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- that many samples was plotted for biopsy (BX) and blood (PAX) samples.
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- The selected batch size, 5, is marked with a dotted vertical line.
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+Number of batches usable in fRMA probe weight learning as a function of
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+ batch size.
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\end_layout
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\end_layout
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\end_inset
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\end_inset
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@@ -6237,7 +6182,8 @@ For batch sizes ranging from 3 to 15, the number of batches with at least
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\end_layout
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\end_layout
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-\begin_layout Standard
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+\begin_layout Plain Layout
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+\align center
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\begin_inset Float figure
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\begin_inset Float figure
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placement tb
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placement tb
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wide false
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wide false
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@@ -6248,6 +6194,9 @@ status collapsed
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/frma-pax-bx/batchsize_samples.pdf
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filename graphics/frma-pax-bx/batchsize_samples.pdf
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+ lyxscale 50
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+ width 100col%
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+ groupId frmatools-subfig
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\end_inset
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\end_inset
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@@ -6266,12 +6215,39 @@ name "fig:batch-size-samples"
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\series bold
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\series bold
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-Effect of batch size selection on number of samples included in fRMA probe
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- weight learning.
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+Number of samples usable in fRMA probe weight learning as a function of
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+ batch size.
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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+\series bold
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:frmatools-batch-size"
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+
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+\end_inset
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+
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+Effect of batch size selection on number of batches and number of samples
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+ included in fRMA probe weight learning.
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\series default
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\series default
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-For batch sizes ranging from 3 to 15, the number of samples included in
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- probe weight training was plotted for biopsy (BX) and blood (PAX) samples.
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+For batch sizes ranging from 3 to 15, the number of batches (a) and samples
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+ (b) included in probe weight training were plotted for biopsy (BX) and
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+ blood (PAX) samples.
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The selected batch size, 5, is marked with a dotted vertical line.
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The selected batch size, 5, is marked with a dotted vertical line.
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\end_layout
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\end_layout
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@@ -6289,28 +6265,18 @@ For batch sizes ranging from 3 to 15, the number of samples included in
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In order to enable use of fRMA to normalize hthgu133pluspm, a custom set
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In order to enable use of fRMA to normalize hthgu133pluspm, a custom set
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of fRMA vectors was created.
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of fRMA vectors was created.
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First, an appropriate batch size was chosen by looking at the number of
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First, an appropriate batch size was chosen by looking at the number of
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- batches and number of samples included as a function of batch size (Figures
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+ batches and number of samples included as a function of batch size (Figure
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\begin_inset CommandInset ref
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\begin_inset CommandInset ref
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LatexCommand ref
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LatexCommand ref
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-reference "fig:batch-size-batches"
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+reference "fig:frmatools-batch-size"
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plural "false"
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plural "false"
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caps "false"
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caps "false"
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noprefix "false"
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noprefix "false"
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\end_inset
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\end_inset
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- and
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:batch-size-samples"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-, respectively).
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+).
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For a given batch size, all batches with fewer samples that the chosen
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For a given batch size, all batches with fewer samples that the chosen
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size must be ignored during training, while larger batches must be randomly
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size must be ignored during training, while larger batches must be randomly
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downsampled to the chosen size.
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downsampled to the chosen size.
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@@ -6357,12 +6323,18 @@ wide false
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sideways false
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sideways false
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status collapsed
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status collapsed
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+\begin_layout Plain Layout
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status collapsed
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+
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/frma-pax-bx/M-BX-violin.pdf
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filename graphics/frma-pax-bx/M-BX-violin.pdf
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lyxscale 40
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lyxscale 40
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- height 80theight%
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+ height 45col%
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groupId m-violin
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groupId m-violin
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\end_inset
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\end_inset
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@@ -6381,14 +6353,80 @@ name "fig:m-bx-violin"
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\end_inset
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\end_inset
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+\series bold
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+Violin plot of inter-normalization log ratios for biopsy samples.
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\begin_inset space \hfill{}
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+\end_inset
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+
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+
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+\begin_inset Float figure
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+wide false
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+sideways false
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+status open
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+
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+\begin_layout Plain Layout
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+\align center
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+\begin_inset Graphics
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+ filename graphics/frma-pax-bx/M-PAX-violin.pdf
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+ lyxscale 40
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+ height 45col%
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+ groupId m-violin
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:m-pax-violin"
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+
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+\end_inset
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+
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+
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+\series bold
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+Violin plot of inter-normalization log ratios for blood samples.
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\end_inset
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+
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\series bold
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\series bold
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Violin plot of log ratios between normalizations for 20 biopsy samples.
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Violin plot of log ratios between normalizations for 20 biopsy samples.
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\series default
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\series default
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-Each of 20 randomly selected biopsy samples was normalized with RMA and
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- with 5 different sets of fRMA vectors.
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- This shows the distribution of log ratios between normalized expression
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- values, aggregated across all 20 arrays.
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+Each of 20 randomly selected samples was normalized with RMA and with 5
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+ different sets of fRMA vectors.
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+ The distribution of log ratios between normalized expression values, aggregated
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+ across all 20 arrays, was plotted for each pair of normalizations.
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\end_layout
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\end_layout
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\end_inset
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\end_inset
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@@ -6439,12 +6477,19 @@ wide false
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sideways false
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status collapsed
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filename graphics/frma-pax-bx/MA-BX-RMA.fRMA.pdf
|
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filename graphics/frma-pax-bx/MA-BX-RMA.fRMA.pdf
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lyxscale 50
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lyxscale 50
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- width 100text%
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+ width 45col%
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groupId ma-frma
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groupId ma-frma
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@@ -6464,13 +6509,57 @@ name "fig:ma-bx-rma-frma"
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\series bold
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\series bold
|
|
-Representative MA plot comparing RMA against fRMA for 20 biopsy samples.
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+RMA vs.
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+ fRMA for biopsy samples.
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+\end_layout
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+\align center
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+\begin_inset Graphics
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+ filename graphics/frma-pax-bx/MA-BX-fRMA.fRMA.pdf
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+ lyxscale 50
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+ width 45col%
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+
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+
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+\end_layout
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+
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+\begin_layout Plain Layout
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+\begin_inset Caption Standard
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+
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+\begin_layout Plain Layout
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:ma-bx-frma-frma"
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+
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+\end_inset
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+
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+
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+\series bold
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+fRMA vs fRMA for biopsy samples.
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\series default
|
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\series default
|
|
-Averages and log ratios were computed for every probe in each of 20 biopsy
|
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|
- samples between RMA normalization and fRMA.
|
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|
|
- Density of points is represented by darkness of shading, and individual
|
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|
- outlier points are plotted.
|
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+Two different fRMA normalizations using vectors from two different batch
|
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+ samplings were compared.
|
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\end_layout
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\end_layout
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\end_inset
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\end_inset
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@@ -6483,7 +6572,8 @@ Averages and log ratios were computed for every probe in each of 20 biopsy
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\end_layout
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\end_layout
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-\begin_layout Standard
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+\begin_layout Plain Layout
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wide false
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sideways false
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sideways false
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@@ -6492,9 +6582,9 @@ status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
|
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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- filename graphics/frma-pax-bx/MA-BX-fRMA.fRMA.pdf
|
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+ filename graphics/frma-pax-bx/MA-PAX-RMA.fRMA.pdf
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lyxscale 50
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lyxscale 50
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- width 100text%
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+ width 45col%
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groupId ma-frma
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groupId ma-frma
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\end_inset
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\end_inset
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@@ -6508,18 +6598,93 @@ status collapsed
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\begin_inset CommandInset label
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|
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LatexCommand label
|
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LatexCommand label
|
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-name "fig:ma-bx-frma-frma"
|
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+name "fig:MA-PAX-rma-frma"
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+
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+\end_inset
|
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+
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+
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+\series bold
|
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|
+RMA vs.
|
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|
+ fRMA for blood samples.
|
|
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|
+\end_layout
|
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+
|
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+\end_inset
|
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+
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+
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+
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+
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+\begin_inset space \hfill{}
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+\end_inset
|
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+
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+\begin_inset Float figure
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+sideways false
|
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+status collapsed
|
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+
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|
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+\begin_layout Plain Layout
|
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|
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+\align center
|
|
|
|
+\begin_inset Graphics
|
|
|
|
+ filename graphics/frma-pax-bx/MA-PAX-fRMA.fRMA.pdf
|
|
|
|
+ lyxscale 50
|
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|
|
+ width 45col%
|
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+ groupId ma-frma
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+
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+\end_inset
|
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+
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+
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|
+\end_layout
|
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+
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|
+\begin_layout Plain Layout
|
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|
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+\begin_inset Caption Standard
|
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+
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+\begin_layout Plain Layout
|
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+\begin_inset CommandInset label
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+LatexCommand label
|
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|
+name "fig:MA-PAX-frma-frma"
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+
|
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|
+\end_inset
|
|
|
|
+
|
|
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+
|
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|
|
+\series bold
|
|
|
|
+fRMA vs fRMA for blood samples.
|
|
|
|
+
|
|
|
|
+\series default
|
|
|
|
+Two different fRMA normalizations using vectors from two different batch
|
|
|
|
+ samplings were compared.
|
|
|
|
+\end_layout
|
|
|
|
+
|
|
|
|
+\end_inset
|
|
|
|
+
|
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+
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+\end_layout
|
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\end_inset
|
|
\end_inset
|
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|
+\end_layout
|
|
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+
|
|
|
|
+\begin_layout Plain Layout
|
|
|
|
+\begin_inset Caption Standard
|
|
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|
+
|
|
|
|
+\begin_layout Plain Layout
|
|
|
|
+
|
|
\series bold
|
|
\series bold
|
|
-Representative MA plot comparing different fRMA vectors for 20 biopsy samples.
|
|
|
|
|
|
+\begin_inset CommandInset label
|
|
|
|
+LatexCommand label
|
|
|
|
+name "fig:Representative-MA-plots"
|
|
|
|
+
|
|
|
|
+\end_inset
|
|
|
|
+
|
|
|
|
+Representative MA plots comparing RMA and custom fRMA normalizations.
|
|
|
|
|
|
\series default
|
|
\series default
|
|
-Averages and log ratios were computed for every probe in each of 20 biopsy
|
|
|
|
- samples between fRMA normalizations using vectors from two different batch
|
|
|
|
- samplings.
|
|
|
|
|
|
+For each plot, 20 samples were normalized using 2 different normalizations,
|
|
|
|
+ and then averages and log ratios were computed between the two different
|
|
|
|
+ normalizations for every probe.
|
|
Density of points is represented by darkness of shading, and individual
|
|
Density of points is represented by darkness of shading, and individual
|
|
outlier points are plotted.
|
|
outlier points are plotted.
|
|
\end_layout
|
|
\end_layout
|
|
@@ -6628,49 +6793,37 @@ noprefix "false"
|
|
well.
|
|
well.
|
|
\end_layout
|
|
\end_layout
|
|
|
|
|
|
|
|
+\begin_layout Subsection
|
|
|
|
+SVA, voom, and array weights improve model fit for methylation array data
|
|
|
|
+\end_layout
|
|
|
|
+
|
|
\begin_layout Standard
|
|
\begin_layout Standard
|
|
\begin_inset Float figure
|
|
\begin_inset Float figure
|
|
wide false
|
|
wide false
|
|
-sideways false
|
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|
|
-status collapsed
|
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|
+sideways true
|
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|
+status open
|
|
|
|
|
|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
|
-\align center
|
|
|
|
-\begin_inset Graphics
|
|
|
|
- filename graphics/frma-pax-bx/M-PAX-violin.pdf
|
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|
- lyxscale 40
|
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|
- height 80theight%
|
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- groupId m-violin
|
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-
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-\end_inset
|
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-
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-
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|
-\end_layout
|
|
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+\begin_inset Float figure
|
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+wide false
|
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+sideways false
|
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|
+status open
|
|
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|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
|
-\begin_inset Caption Standard
|
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+\align center
|
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|
|
+\begin_inset Flex TODO Note (inline)
|
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|
+status open
|
|
|
|
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|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
|
-\begin_inset CommandInset label
|
|
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|
-LatexCommand label
|
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|
-name "fig:m-pax-violin"
|
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-
|
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|
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|
+Fix axis labels:
|
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|
+\begin_inset Quotes eld
|
|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
-
|
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|
-\series bold
|
|
|
|
-Violin plot of log ratios between normalizations for 20 blood samples.
|
|
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|
-
|
|
|
|
-\series default
|
|
|
|
-Each of 20 randomly selected blood samples was normalized with RMA and with
|
|
|
|
- 5 different sets of fRMA vectors.
|
|
|
|
- This shows the distribution of log ratios between normalized expression
|
|
|
|
- values, aggregated across all 20 arrays.
|
|
|
|
-\end_layout
|
|
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|
-
|
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|
|
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|
+log2 M-value
|
|
|
|
+\begin_inset Quotes erd
|
|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
-
|
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|
|
|
+ is redundant because M-values are already log scale
|
|
\end_layout
|
|
\end_layout
|
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|
\end_inset
|
|
\end_inset
|
|
@@ -6678,19 +6831,13 @@ Each of 20 randomly selected blood samples was normalized with RMA and with
|
|
|
|
|
|
\end_layout
|
|
\end_layout
|
|
|
|
|
|
-\begin_layout Standard
|
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|
|
-\begin_inset Float figure
|
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-wide false
|
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|
-sideways false
|
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|
|
-status collapsed
|
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|
-
|
|
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|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
|
\align center
|
|
\align center
|
|
\begin_inset Graphics
|
|
\begin_inset Graphics
|
|
- filename graphics/frma-pax-bx/MA-PAX-RMA.fRMA.pdf
|
|
|
|
- lyxscale 50
|
|
|
|
- width 100text%
|
|
|
|
- groupId ma-frma
|
|
|
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|
|
+ filename graphics/methylvoom/unadj.dupcor/meanvar-trends-PAGE1-CROP-RASTER.png
|
|
|
|
+ lyxscale 15
|
|
|
|
+ width 30col%
|
|
|
|
+ groupId voomaw-subfig
|
|
|
|
|
|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
@@ -6701,21 +6848,16 @@ status collapsed
|
|
\begin_inset Caption Standard
|
|
\begin_inset Caption Standard
|
|
|
|
|
|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
|
|
|
+
|
|
|
|
+\series bold
|
|
\begin_inset CommandInset label
|
|
\begin_inset CommandInset label
|
|
LatexCommand label
|
|
LatexCommand label
|
|
-name "fig:MA-PAX-rma-frma"
|
|
|
|
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|
+name "fig:meanvar-basic"
|
|
|
|
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|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
-
|
|
|
|
-\series bold
|
|
|
|
-Representative MA plot comparing RMA against fRMA for 20 blood samples.
|
|
|
|
|
|
+Mean-variance trend for analysis A.
|
|
|
|
|
|
-\series default
|
|
|
|
-Averages and log ratios were computed for every probe in each of 20 blood
|
|
|
|
- samples between RMA normalization and fRMA.
|
|
|
|
- Density of points is represented by darkness of shading, and individual
|
|
|
|
- outlier points are plotted.
|
|
|
|
\end_layout
|
|
\end_layout
|
|
|
|
|
|
\end_inset
|
|
\end_inset
|
|
@@ -6723,28 +6865,25 @@ Averages and log ratios were computed for every probe in each of 20 blood
|
|
|
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|
\end_layout
|
|
\end_layout
|
|
|
|
|
|
-\begin_layout Plain Layout
|
|
|
|
|
|
+\end_inset
|
|
|
|
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|
-\end_layout
|
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|
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+\begin_inset space \hfill{}
|
|
\end_inset
|
|
\end_inset
|
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|
|
|
|
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|
-\end_layout
|
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|
-
|
|
|
|
-\begin_layout Standard
|
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|
\begin_inset Float figure
|
|
\begin_inset Float figure
|
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wide false
|
|
wide false
|
|
sideways false
|
|
sideways false
|
|
-status collapsed
|
|
|
|
|
|
+status open
|
|
|
|
|
|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
|
\align center
|
|
\align center
|
|
\begin_inset Graphics
|
|
\begin_inset Graphics
|
|
- filename graphics/frma-pax-bx/MA-PAX-fRMA.fRMA.pdf
|
|
|
|
- lyxscale 50
|
|
|
|
- width 100text%
|
|
|
|
- groupId ma-frma
|
|
|
|
|
|
+ filename graphics/methylvoom/unadj.dupcor.sva.aw/meanvar-trends-PAGE1-CROP-RASTER.png
|
|
|
|
+ lyxscale 15
|
|
|
|
+ width 30col%
|
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|
+ groupId voomaw-subfig
|
|
|
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|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
@@ -6755,22 +6894,15 @@ status collapsed
|
|
\begin_inset Caption Standard
|
|
\begin_inset Caption Standard
|
|
|
|
|
|
\begin_layout Plain Layout
|
|
\begin_layout Plain Layout
|
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+
|
|
|
|
+\series bold
|
|
\begin_inset CommandInset label
|
|
\begin_inset CommandInset label
|
|
LatexCommand label
|
|
LatexCommand label
|
|
-name "fig:MA-PAX-frma-frma"
|
|
|
|
|
|
+name "fig:meanvar-sva-aw"
|
|
|
|
|
|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
-
|
|
|
|
-\series bold
|
|
|
|
-Representative MA plot comparing different fRMA vectors for 20 blood samples.
|
|
|
|
-
|
|
|
|
-\series default
|
|
|
|
-Averages and log ratios were computed for every probe in each of 20 blood
|
|
|
|
- samples between fRMA normalizations using vectors from two different batch
|
|
|
|
- samplings.
|
|
|
|
- Density of points is represented by darkness of shading, and individual
|
|
|
|
- outlier points are plotted.
|
|
|
|
|
|
+Mean-variance trend for analysis B.
|
|
\end_layout
|
|
\end_layout
|
|
|
|
|
|
\end_inset
|
|
\end_inset
|
|
@@ -6781,49 +6913,41 @@ Averages and log ratios were computed for every probe in each of 20 blood
|
|
\end_inset
|
|
\end_inset
|
|
|
|
|
|
|
|
|
|
-\end_layout
|
|
|
|
-
|
|
|
|
-\begin_layout Standard
|
|
|
|
-\begin_inset ERT
|
|
|
|
-status collapsed
|
|
|
|
|
|
+\begin_inset space \hfill{}
|
|
|
|
+\end_inset
|
|
|
|
|
|
-\begin_layout Plain Layout
|
|
|
|
|
|
|
|
|
|
+\begin_inset Float figure
|
|
|
|
+wide false
|
|
|
|
+sideways false
|
|
|
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+status open
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-\backslash
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+\begin_layout Plain Layout
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+\align center
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+ filename graphics/methylvoom/unadj.dupcor.sva.voomaw/meanvar-trends-PAGE2-CROP-RASTER.png
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+ lyxscale 15
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\end_layout
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\end_layout
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-\begin_layout Subsection
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-SVA, voom, and array weights improve model fit for methylation array data
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-\end_layout
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-
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-log2 M-value
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-\begin_inset Quotes erd
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+\series bold
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+\begin_inset CommandInset label
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+LatexCommand label
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+name "fig:meanvar-sva-voomaw"
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+
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\end_inset
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- is redundant because M-values are already log scale
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+Mean-variance trend after voom modeling in analysis C.
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\end_layout
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\end_layout
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\end_inset
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\end_inset
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@@ -6831,14 +6955,6 @@ log2 M-value
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\end_layout
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\end_layout
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-\begin_layout Plain Layout
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-\align center
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- filename graphics/methylvoom/unadj.dupcor/meanvar-trends-PAGE1-CROP-RASTER.png
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- lyxscale 15
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- width 100col%
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@@ -6850,13 +6966,7 @@ log2 M-value
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\series bold
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\series bold
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-name "fig:meanvar-basic"
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-\end_inset
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-
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-Mean-variance trend for analysis A.
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+Mean-variance trend modeling in methylation array data.
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\series default
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\series default
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The log2(standard deviation) for each probe is plotted against the probe's
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The log2(standard deviation) for each probe is plotted against the probe's
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@@ -6865,6 +6975,7 @@ The log2(standard deviation) for each probe is plotted against the probe's
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Density of points is also indicated by the dark blue contour lines.
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Density of points is also indicated by the dark blue contour lines.
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The prior variance trend estimated by eBayes is shown in light blue, while
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The prior variance trend estimated by eBayes is shown in light blue, while
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the lowess trend of the points is shown in red.
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the lowess trend of the points is shown in red.
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+
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\end_layout
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\end_layout
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\end_inset
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@@ -6921,62 +7032,6 @@ noprefix "false"
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that is only observable at sites with varying methylation.
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that is only observable at sites with varying methylation.
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\end_layout
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-
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-\begin_layout Plain Layout
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- filename graphics/methylvoom/unadj.dupcor.sva.aw/meanvar-trends-PAGE1-CROP-RASTER.png
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- lyxscale 15
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- width 100col%
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-\begin_layout Plain Layout
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-\begin_inset Caption Standard
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-
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-\series bold
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-\begin_inset CommandInset label
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-LatexCommand label
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-name "fig:meanvar-sva-aw"
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-
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-\end_inset
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-
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-Mean-variance trend for analysis B.
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-
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-\series default
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-Interpretation is as in Figure
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:meanvar-basic"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-.
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-
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\begin_layout Standard
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\begin_layout Standard
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In Figure
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In Figure
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\begin_inset CommandInset ref
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\begin_inset CommandInset ref
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@@ -7012,62 +7067,6 @@ absorbed
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variation has no systematic component: probes with extreme M-values are
|
|
variation has no systematic component: probes with extreme M-values are
|
|
uniformly more variable across all samples, as expected.
|
|
uniformly more variable across all samples, as expected.
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-\end_layout
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-
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-\begin_layout Standard
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-\begin_inset Float figure
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-wide false
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-sideways false
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-status collapsed
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-
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-\begin_layout Plain Layout
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-\begin_inset Graphics
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- filename graphics/methylvoom/unadj.dupcor.sva.voomaw/meanvar-trends-PAGE2-CROP-RASTER.png
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- lyxscale 15
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- width 100col%
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- groupId raster-600ppi
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-\begin_layout Plain Layout
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-\begin_inset Caption Standard
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-\begin_layout Plain Layout
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-LatexCommand label
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-name "fig:meanvar-sva-voomaw"
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-
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-\end_inset
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-
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-Mean-variance trend after voom modeling in analysis C.
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-
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-\series default
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-Interpretation is as in Figure
|
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-\begin_inset CommandInset ref
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-LatexCommand ref
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-reference "fig:meanvar-basic"
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-plural "false"
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-caps "false"
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-noprefix "false"
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-
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-\end_inset
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-
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-.
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-\end_layout
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-\end_inset
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-
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\end_layout
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\end_layout
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\begin_layout Standard
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\begin_layout Standard
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@@ -7673,7 +7672,7 @@ noprefix "false"
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\begin_inset Float table
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\begin_inset Float table
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wide false
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wide false
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sideways false
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sideways false
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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@@ -8077,22 +8076,6 @@ Maybe include the PCA plots before/after SVA effect subtraction?
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\end_inset
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\end_inset
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-\end_layout
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-
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-\begin_layout Standard
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-\begin_inset ERT
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-status collapsed
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-
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\begin_layout Section
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\begin_layout Section
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@@ -8923,12 +8906,15 @@ literal "false"
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways false
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sideways false
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/Globin Paper/figure1 - globin-fractions.pdf
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filename graphics/Globin Paper/figure1 - globin-fractions.pdf
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+ lyxscale 50
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+ width 100col%
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+ groupId colwidth
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\end_inset
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\end_inset
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@@ -8995,7 +8981,7 @@ Fraction of genic reads in each sample aligned to non-globin genes, with
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placement p
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placement p
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wide false
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wide false
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sideways true
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sideways true
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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@@ -9665,12 +9651,15 @@ Remove redundant titles from figures
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways false
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sideways false
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/Globin Paper/figure2 - aveLogCPM-colored.pdf
|
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filename graphics/Globin Paper/figure2 - aveLogCPM-colored.pdf
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+ lyxscale 50
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+ width 100col%
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+ groupId colwidth
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\end_inset
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@@ -9768,12 +9757,15 @@ noprefix "false"
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways false
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sideways false
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/Globin Paper/figure3 - detection.pdf
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filename graphics/Globin Paper/figure3 - detection.pdf
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+ lyxscale 50
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+ width 100col%
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+ groupId colwidth
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@@ -9892,12 +9884,15 @@ One potential worry is that the globin blocking protocol could perturb the
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways false
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sideways false
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/Globin Paper/figure4 - maplot-colored.pdf
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filename graphics/Globin Paper/figure4 - maplot-colored.pdf
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+ lyxscale 50
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+ width 100col%
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@@ -10025,12 +10020,15 @@ noprefix "false"
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\begin_inset Float figure
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\begin_inset Float figure
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wide false
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wide false
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sideways false
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sideways false
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-status open
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+status collapsed
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\begin_layout Plain Layout
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\begin_layout Plain Layout
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\align center
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\align center
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\begin_inset Graphics
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\begin_inset Graphics
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filename graphics/Globin Paper/figure5 - corrplot.pdf
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filename graphics/Globin Paper/figure5 - corrplot.pdf
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+ lyxscale 50
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@@ -10143,7 +10141,7 @@ More differentially expressed genes are detected with globin blocking
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\begin_inset Float table
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wide false
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wide false
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sideways false
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sideways false
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\begin_layout Plain Layout
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\align center
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