Ryan C. Thompson b6928049d1 Add Rmd and RDS files for RNA-seq lesson 5 anos atrás
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450k 0df8e97622 Add output files so they show up on GH pages 8 anos atrás
CD4 1d4a0eb2a8 Add current draft of thesis to examples 5 anos atrás
Pathways 90ddb01b0a Generate all index.html files 8 anos atrás
Teaching b6928049d1 Add Rmd and RDS files for RNA-seq lesson 5 anos atrás
fRMA 90ddb01b0a Generate all index.html files 8 anos atrás
globin 0df8e97622 Add output files so they show up on GH pages 8 anos atrás
Advanced RNA-seq Analysis.pdf 997ff2d4fc Initial CV draft 5 anos atrás
C. elegans PCA.pdf 60d111c398 Initial import into git 9 anos atrás
Classifier Math Write-up.pdf 60d111c398 Initial import into git 9 anos atrás
DGE Presentation.pdf 60d111c398 Initial import into git 9 anos atrás
README.mkdn 1d4a0eb2a8 Add current draft of thesis to examples 5 anos atrás
Reproducible Workflow Presentation.pdf 1688441330 Add reproducible workflow presentation 8 anos atrás
blockbuster-pipeline.R 60d111c398 Initial import into git 9 anos atrás
blockbuster-pipeline.R.html 317743fad5 Convert R files to html 8 anos atrás
defense-slides.pdf 1d4a0eb2a8 Add current draft of thesis to examples 5 anos atrás
index.html 1d4a0eb2a8 Add current draft of thesis to examples 5 anos atrás
mdsplots-multidim.pdf 60d111c398 Initial import into git 9 anos atrás
thesis-final.pdf 1d4a0eb2a8 Add current draft of thesis to examples 5 anos atrás

README.mkdn

## My thesis

- [My thesis](thesis-final.pdf): *Bioinformatic analysis of complex,
high-throughput genomic and epigenomic data in the context of CD4⁺
T-cell differentiation and diagnosis and treatment of transplant
rejection*
- [Slides for my defense talk](defense-slides.pdf): The slides for
my dissertation defense, mainly covering Chapter 2 of my thesis

## Interesting sub-folders

Each folder showcases a different project or aspect of my work in the
Salomon lab.

- [`Teaching`](Teaching): Teaching materials for my lecture and lab on
introductory RNA-seq analysis
- [`CD4`](CD4): Examples relating to my CD4 memory RNA-seq & ChIP-seq
project
- [`450k`](450k): Examples relating to analysis of Illumina 450k DNA
methylation arrays
- [`Pathways`](Pathways): Examples of gene set and pathway analysis
for RNA-seq data
- [`globin`](globin): Examples of my work in demonstrating the
performance of a new globin blocking protocol for RNA-seq of blood
tissues
- [`fRMA`](fRMA): Examples and code from my work creating a custom
frozen RMA reference to provide a stable normalization for machine
learning applications

## Presentations

- [`DGE Presentation.pdf`](DGE Presentation.pdf): comparing edgeR,
DESeq, and limma on both a conceptual and practical level
- [`Advanced RNA-seq Analysis.pdf`](Advanced RNA-seq Analysis.pdf): On
the advanced features provided by limma for RNA-seq analysis
- [`Reproducible Workflow Presentation.pdf`](Reproducible Workflow
Presentation.pdf): Showcases the use of
[Snakemake](https://snakemake.readthedocs.io/en/stable/) for
building a reproducible analysis

## Other files

- [`Classifier Math Write-up.pdf`](Classifier Math Write-up.pdf): A
short write-up to explain the mathematical principles behind the
classifier method used in a machine learning project
- [`blockbuster-pipeline.R.html`](blockbuster-pipeline.R.html): R code
for clustering small RNA reads into clusters
using
[Blockbuster](http://hoffmann.bioinf.uni-leipzig.de/LIFE/blockbuster.html)
- [`mdsplots-multidim.pdf`](mdsplots-multidim.pdf): A PCA plot of RNA-seq
data for *C. elegans* samples with and without a drug that retards
the aging process, demonstrating smaller age-related changes with
the drug
(for [this paper](https://elifesciences.org/content/4/e08833))