resume/ryan_thompson_resume.lyx

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Ryan C.
 Thompson, Ph.
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D.
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https://darwinawardwinner.github.io/resume/ryan_thompson_resume.pdf
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\begin_layout CV Name
Ryan C.
 Thompson, Ph.
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D.
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\begin_layout Contact Address
8656 Via Mallorca
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Unit G
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La Jolla, CA 92037
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\begin_layout Phone Number
(908)
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922-7470
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\begin_layout Email
rct@thompsonclan.org
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\begin_layout Website
https://github.com/DarwinAwardWinner
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\begin_layout Section
Summary
\end_layout

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Seeking what kind of job?
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\begin_layout Itemize
Accomplished, experienced computational biologist with deep knowledge of
 biology, mathematics, statistics, and programming.
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\begin_layout Itemize
11 years of bioinformatics experience answering a wide range of complex
 biological questions through analysis of large NGS- and microarray-based
 whole transcriptome and epigenetic data sets.
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Focus on sound statistical analysis, effective data visualization and communicat
ion, and making expert knowledge and successful analysis methods available
 as re-usable software tools.
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Work in machine learning?
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Supportive team member and experienced mentor with a strong ability to help
 others connect biological, statistical, and computational understanding.
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Education
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2012—2019
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Ph.
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D.
 in Bioinformatics
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, 
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The Scripps Research Institute
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, La Jolla, California 
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Dissertation: 
\emph on

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LatexCommand href
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Update this link to one in the resume repo when possible
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Advisor: 
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name "Andrew Su"
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2005—2009
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B.
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S.
 in Biology with High Distinction; B.
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A.
 in Mathematics
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University of Virginia
\series default
, Charlottesville, Virginia 
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Undergraduate thesis: 
\emph on

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Advisor: 
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Research Experience
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Salomon & Su Labs, The Scripps Research Institute 
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 La Jolla, CA
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 2012 - 2019
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Contact: Dr.
 Andrew Su, 
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Lacking impact statement.
 Say what I did and what the impact was.
 Also say methodology applied.
 First sentence: problem statement.
 2nd sentence: methodology applied.
 3rd sentence: impact/result/take-home
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Created an open source, reproducible workflow to analyze a large multi-omics
 next-gen sequencing dataset of 220 RNA-seq and ChIP-seq samples to reveal
 coordinated changes in histone marks and gene expression during CD4
\begin_inset Formula $^{+}$
\end_inset

 T-cell activation as well as epigenetic convergence between naive and memory
 cells after activation.
 
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literal "true"

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.
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Significantly improved cross-validated performance of machine learning classifie
r for identifying biomarkers of transplant rejection by developing appropriate
 single-sample microarray normalization procedures
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literal "true"

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, including training a custom set of frozen RMA normalization vectors.
 Classifier is currently being developed into a clinical test for transplant
 dysfunction.
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Performed a comprehensive comparative evaluation of over 20 subtly different
 statistical models for differential methylation in Illumina 450k arrays,
 selecting a model that best explained the observed sources and trends of
 variation in the data, including cross-domain application of a method originall
y designed for RNA-seq data.
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Evaluated and optimized cost and performance of custom protocol for RNA-seq
 of human and primate blood samples while minimizing nuisance globin reads.
 Increased yield of useful reads nearly 2-fold.
 
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Investigated effects of life-span-extending drug on worm gene expression
 over time revealing that the drug retards age-related 
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transcriptional drift
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, preserving a youthful phenotype at the molecular level.
 
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Implemented a systems biology tool to analyze and efficiently present and
 summarize differential expression for multiple gene set & pathway methods
 run on multiple pathway databases, as well as differential expression of
 individual genes within each pathway.
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\begin_layout Itemize
Performed comparative analysis of multiple differential expression statistical
 models to define best practice for optimal sensitivity while maintaining
 false positive control.
 Presented on theoretical and practical similarities and differences between
 methods.
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\begin_layout Itemize
Taught basic RNA-seq theory and practical analysis for the graduate-level
 introductory bioinformatics course.
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Adapted common normalization methods from RNA-seq to improve performance
 in analysis of RASL-seq experiments.
 
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Active member of the Bioconductor community since 2012 and contributing
 developer for several Bioconductor packages.
 
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Mentored 3 coworkers in learning bioinformatics, one from a programming
 background and the other from a biology background.
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Summer Internship, Informatics IT, Merck & Co.
 
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 Boston, MA
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 2011
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Contact: Adnan Derti, 
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Built a transcriptome assembly and quantification pipeline using Cufflinks,
 including fully-automated cluster job control for high-throughput reproducible
 analysis, and presented a conceptual overview of Cufflinks' assembly and
 quantification algorithms to help the team understand Cufflinks.
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\begin_layout Itemize
Assisted in a molecular genetics study to evaluate peformance of two variant
 calling algorithms in detection of causal mutations in antibiotic-resistant
 bacterial genomes.
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Gaasterland Lab, UCSD Bioinformatcs 
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 La Jolla, CA
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 2010—2012
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Contact: Terry Gaasterland, 
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Designed and implemented Deloxer, a critical software step in a new Illumina
 mate-pair sequencing protocol using Cre recombination.
 Deloxer is published
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literal "true"

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 and now in use in several labs around the world.
 
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; 
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\begin_layout Itemize
Performed a molecular genetics study to find potential causal mutations
 for 
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target "https://en.wikipedia.org/wiki/Black_rhinoceros#Threats"
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 in critically endangered black rhinoceros by 
\emph on
de novo
\emph default
 assembly of transcriptomes for black rhino and closely-related white rhino.
 Developed a custom pipeline to match up ortholog gene pairs, discover single-nu
cleotide differences between them, and functionally annotate these differences,
 and delivered a list of potential causal variants to collaborators for
 follow-up.
 
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Helped design & implement a large-scale high-throughput exome sequencing
 pipeline for SNP discovery and functional annotation, including QC and
 validation of on-target coverage depth and reproducibility of coverage.
 
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LatexCommand href
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; 
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; 
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Created a fully-automated pipeline to produce quality-control metrics and
 plots for Illumina high-throughput sequencing data for early identification
 of failed runs or samples.
 
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\begin_layout Itemize
Investigated the binding motif specificity of ZASC1 transcription factor
 in mouse T-cells using Affymetrix expression microarrays in ZASC1 siRNA
 knockdown experiment.
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Analyzed miRNA target predictions using GO & KEGG grouping to identify target
 pathways of autophagy-related miRNAs for biological validation.
 
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Timko Lab, U.
 of Virginia Biology 
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 Charlottesville, VA
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 2007—2009
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Contact: Paul J.
 Rushton, 
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\begin_layout Itemize
Undergraduate thesis: Designed and implemented Contig Farmer, an algorithm
 for efficient selective contig assembly starting from “seed” sequences
 of interest, and used Contig Farmer to accelerate transcription factor
 gene discovery in cowpea and tobacco shotgun genomic sequence data
\begin_inset CommandInset citation
LatexCommand cite
key "Thompson2009"
literal "true"

\end_inset

.
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\begin_layout Itemize
Investigated transcription factors mediating plant stress response using
 expression microarray time-course, and refined the custom microarray design
 using data from previous runs to identify and eliminate uninformative probes,
 yielding an improved design for future studies.
 
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\end_inset


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\begin_layout Itemize
Acted as interpreter to explain complex biological concepts to programmers
 and explain complex computational problems to biologists.
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\begin_layout Section
Skills
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\begin_layout Description
Computing
\begin_inset space ~
\end_inset

Skills R/BioConductor, Python, Perl, Lisp, Java, C#, C++; Git version control;
 Remote UNIX system administration & software compilation, computing cluster
 job management & parallel computation
\begin_inset Newline newline
\end_inset


\size small
Public code: 
\begin_inset CommandInset href
LatexCommand href
target "https://github.com/DarwinAwardWinner"
literal "false"

\end_inset

 
\begin_inset Newline newline
\end_inset

Stack Overflow profile: 
\begin_inset CommandInset href
LatexCommand href
target "http://stackoverflow.com/users/125921"
literal "false"

\end_inset

 
\end_layout

\begin_layout Description
Statistics,
\begin_inset space ~
\end_inset

Machine
\begin_inset space ~
\end_inset

Learning,
\begin_inset space ~
\end_inset

&
\begin_inset space ~
\end_inset

Data
\begin_inset space ~
\end_inset

Analysis Multi-omics NGS & microarray analysis, multi-factor linear and
 generalized linear regression, experimental design and parametrization,
 empirical Bayesian methods, predictive modeling of clinical outcomes, machine
 learning classifier training & validation, survival analysis, data visualizatio
n & presentation, reproducible research practices
\end_layout

\begin_layout Description
Wet
\begin_inset space ~
\end_inset

Lab: PCR, molecular cloning, recombinant protein purification, epitope mapping,
 site-directed mutagenesis, 2D gels, real-time PCR, and associated data
 analysis 
\end_layout

\begin_layout Description
Languages: English: native speaker; German: written and spoken
\end_layout

\begin_layout Section
Other Work Experience
\end_layout

\begin_layout Subsection
Computing Advisor & Help Desk, U.
\begin_inset space \thinspace{}
\end_inset

Va.
 IT Dept.
 
\begin_inset space \hfill{}
\end_inset

 Charlottesville, VA 
\begin_inset space \qquad{}
\end_inset

 2005—2007 
\end_layout

\begin_layout Itemize
Provided support via phone and on-site for students, faculty, and staff
 having problems with computers, phone system, network access, malware,
 hardware setup, and university web services
\end_layout

\begin_layout Itemize
Tasks included support for university-provided software, virus removal,
 iPod recovery, printer setup and repair, diagnosis of hardware malfunctions,
 and data recovery from failing hard disks
\end_layout

\begin_layout Subsection
Summer Sailing Instructor, Raritan Yacht Club 
\begin_inset space \hfill{}
\end_inset

 Perth Amboy, NJ 
\begin_inset space \qquad{}
\end_inset

 2006—2009 
\end_layout

\begin_layout Itemize
Instructed children ages 8-18 in sailing skills, safety, seamanship, knots,
 rigging & de-rigging boats, navigation, and racing strategy and technique,
 with an emphasis on building character and self-reliance
\end_layout

\begin_layout Itemize
Ensured safety of students and staff by maintaining boats and equipment
 in good repair, by being vigilant to traffic and hazards on a busy waterway,
 and by communicating and coordinating efficiently with other staff
\end_layout

\begin_layout Itemize
Helped organize, run, and referee several regattas per season for students
 from RYC and neighboring yacht clubs
\end_layout

\begin_layout Section
Peer-reviewed Publications
\end_layout

\begin_layout Standard
\begin_inset Note Note
status open

\begin_layout Plain Layout
The following command suppresses the bibliography's own title, since we
 have one above that's formatted the way we want.
\end_layout

\end_inset


\end_layout

\begin_layout Standard
\begin_inset ERT
status open

\begin_layout Plain Layout


\backslash
begingroup 
\end_layout

\begin_layout Plain Layout


\backslash
renewcommand{
\backslash
section}[2]{}%
\end_layout

\end_inset


\end_layout

\begin_layout Standard
\begin_inset CommandInset bibtex
LatexCommand bibtex
btprint "btPrintAll"
bibfiles "mypubs-PROCESSED"
options "plainurlyr-rev"

\end_inset


\end_layout

\begin_layout Standard
\begin_inset ERT
status open

\begin_layout Plain Layout


\backslash
endgroup
\end_layout

\end_inset


\end_layout

\begin_layout Section
Other Works
\end_layout

\begin_layout Standard
\begin_inset ERT
status open

\begin_layout Plain Layout


\backslash
begingroup 
\end_layout

\begin_layout Plain Layout


\backslash
renewcommand{
\backslash
section}[2]{}%
\end_layout

\end_inset


\end_layout

\begin_layout Standard
\begin_inset CommandInset bibtex
LatexCommand bibtex
btprint "btPrintAll"
bibfiles "myunpubs-PROCESSED"
options "plainurlyr-rev"

\end_inset


\end_layout

\begin_layout Standard
\begin_inset ERT
status open

\begin_layout Plain Layout


\backslash
endgroup
\end_layout

\end_inset


\end_layout

\begin_layout Standard
\begin_inset Note Note
status open

\begin_layout Plain Layout
Add section on talks
\end_layout

\end_inset


\end_layout

\end_body
\end_document