## Load packages library(openxlsx) library(affy) library(frma) ## Load the fRMA data package and then load the data library(DSalomon.PAX.hthgu133pluspmfrmavecs) data(DSalomon.PAX.hthgu133pluspmfrmavecs) ## Get the sample table and list of CEL files cel.dir <- "Training Data/01 - CTOT08 ABECASSIS PAX Samples" ## Read the first xlsx file in the directory, which is a spreadsheet ## containing all of the CEL file names sample.table <- read.xlsx(list.files(cel.dir, pattern=glob2rx("*.xlsx"), full.names=TRUE)[1]) cel.files <- file.path(cel.dir, sample.table$Filename) ## Read the data from the CEL files affy <- ReadAffy(filenames=cel.files, phenoData=sample.table) ## Apply fRMA eset <- frma(affy, input.vecs=DSalomon.PAX.hthgu133pluspmfrmavecs) ## Extract expression matrix expr <- exprs(eset) ## Extract sample table sample.table <- pData(eset)