Browse Source

Add newer ChIP QC plots to examples

Ryan C. Thompson 9 years ago
parent
commit
e7049bac6a

BIN
examples/Salomon/CD4/CCF-plots.pdf


BIN
examples/Salomon/CD4/H3K4me3 Selected Sample 10KB Bin MA Plots.pdf


BIN
examples/Salomon/CD4/H3K4me3-normfactors.pdf


BIN
examples/Salomon/CD4/H3K4me3-window-abundance-vs-peaks.pdf


+ 37 - 1
examples/Salomon/CD4/README.mkdn

@@ -1,6 +1,9 @@
 This is a series of example plots and tables from a combined
 This is a series of example plots and tables from a combined
 RNA-seq/ChIP-seq study on differences between naive and memory T-cell
 RNA-seq/ChIP-seq study on differences between naive and memory T-cell
-activation.
+activation. You can view the (old and messy) code for these plots
+[here][1].
+
+[1]: https://github.com/DarwinAwardWinner/cd4-histone-paper-code
 
 
 - [`p-value distributions.pdf`](p-value distributions.pdf) is a series
 - [`p-value distributions.pdf`](p-value distributions.pdf) is a series
   of p-value histograms for each of the contrasts tested. A contrast
   of p-value histograms for each of the contrasts tested. A contrast
@@ -19,6 +22,11 @@ activation.
   studied. This was used to determine the "promoter radius" for read
   studied. This was used to determine the "promoter radius" for read
   counting. Notably, the three histone marks do not all have the same
   counting. Notably, the three histone marks do not all have the same
   promoter radius.
   promoter radius.
+- [`rnaseq-MDSPlots.pdf`](rnaseq-MDSPlots.pdf) shows a series of MDS
+  plots (similar to PCA plots) before and after correction of a known
+  batch effect. Note the implausible zigzag-shaped progression over
+  time before correction, compared to the more plausible cyclic time
+  progression after.
 - [`rnaseq-edgeR-vs-limma.pdf`](rnaseq-edgeR-vs-limma.pdf) and
 - [`rnaseq-edgeR-vs-limma.pdf`](rnaseq-edgeR-vs-limma.pdf) and
   [`rnaseq-limma-weighted-vs-uw.pdf`](rnaseq-limma-weighted-vs-uw.pdf)
   [`rnaseq-limma-weighted-vs-uw.pdf`](rnaseq-limma-weighted-vs-uw.pdf)
   show comparisons of p-values for all genes in each contrast of the
   show comparisons of p-values for all genes in each contrast of the
@@ -27,3 +35,31 @@ activation.
   sample quality weights.
   sample quality weights.
 - [`rnaseq-maplots-limma-sampleweights.pdf`](rnaseq-maplots-limma-sampleweights.pdf)
 - [`rnaseq-maplots-limma-sampleweights.pdf`](rnaseq-maplots-limma-sampleweights.pdf)
   shows the MA plot for each contrast of the RNA-seq data
   shows the MA plot for each contrast of the RNA-seq data
+
+
+There are also some plots from an in-progress analysis of the same
+data based on sliding windows, rather than just analyzing promoter
+regions. You can view the code for generating these plots [here][2].
+
+[2]: https://github.com/DarwinAwardWinner/CD4-csaw
+
+- [`CCF-plots.pdf`](CCF-plots.pdf) shows the cross-correlation
+  functions of several different histone marks, at several different
+  levels of smoothing. This plot is used to determine the fragment
+  size.
+- [`site-profile-plots.pdf`](site-profile-plots.pdf) shows plots of
+  the relative coverage depth profiles around local coverage maxima.
+  This plot is used to determine the footprint size of the protein
+  being imunoprecipitated. Since this is histone mark data, the
+  footprint size should match the size of a nucleosome, about 147 bp.
+- [`H3K4me3-window-abundance-vs-peaks.pdf`](H3K4me3-window-abundance-vs-peaks.pdf)
+  shows the association between peak overlap status and abundance for
+  all windows in the genome. As expected, windows that overlap a
+  called peak tend to have a higher abundance than other windows.
+- [`H3K4me3 Selected Sample 10KB Bin MA Plots.pdf`](H3K4me3 Selected
+  Sample 10KB Bin MA Plots.pdf) shows selected MA plots between
+  samples demonstrating the effects of several different potential
+  normalization methods.
+- [`H3K4me3-normfactors.pdf`](H3K4me3-normfactors.pdf) shows the
+  associations between normalization factor and experimental variables
+  for several different normalization methods.

BIN
examples/Salomon/CD4/rnaseq-MDSPlots.pdf


BIN
examples/Salomon/CD4/site-profile-plots.pdf