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@@ -24,9 +24,6 @@ reduction protocol that was designed for cynomolgus monkeys.
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- [`cyno-vs-hg19.pdf`](cyno-vs-hg19.pdf) shows excellent correlation
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between total read counts on the cyno and human genomes, indicating
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that the cyno annotation is reasonably complete.
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-- [`maplot.pdf`](maplot.pdf) shows an MA plot of the effects of globin
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- reduction. Notably, there are real and significant effects on
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- non-globin genes, but they are consistent and predictable.
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- [`pval-comparisons.pdf`](pval-comparisons.pdf) shows the comparison
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between p-values from edgeR, limma-voom, and DESeq2 on the exact
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same differential expression test. Surprisingly, despite the
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@@ -34,3 +31,19 @@ reduction protocol that was designed for cynomolgus monkeys.
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close agreement. DESeq2 id overly liberal because it does not
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account for the negative estimation bias in negative-binomial
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dispersions.
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+
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+# Publication plots
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+
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+These are the figures from an upcoming publication on the developed
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+globin blocking method.
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+
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+- [Figure 1](figure1 - globin-fractions.pdf): Globin blocking (GB)
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+ substantially increases yield and consistency of non-globin reads.
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+- [Figure 2](figure2 - aveLogCPM-colored.pdf): GB lowers the noise
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+ floor and increases the distance between noise floor and signal.
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+- [Figrue 3](figure3 - detection.pdf): GB allows detection of more
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+ genes at any abundance threshold.
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+- [Figure 4](figure4 - maplot-colored.pdf): GB has small but
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+ systematic effects on other genes' measured expression levels.
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+- [Figure 5](figure5 - corrplot.pdf): GB Significantly increases
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+ correlation between libraries.
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